ORIGINAL ARTICLE |
J Korean Med. 2012;33(4): 42-49.
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Effects of Red Ginseng-Ejung-tang Water Extract on Production in LPS-induced Mouse Macrophages |
Wansu Park |
Dept. of Pathology, College of Korean Medicine, Gachon University |
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Corresponding Author:
Wansu Park ,Tel: +82-31-750-8821, Fax: +82-31-750-8821, Email: hangl98@naver.com |
Received: September 8, 2012; Accepted: December 7, 2012. |
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ABSTRACT |
Objectives: The purpose of this study was to investigate effects of Red Ginseng-Ejung-tang Water Extract (ER) on cytokine production in RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS).
Methods: Levels of various cytokines such as interleukin (IL)-6, IL-10, IL-2, IL-12p70, vascular endothelial growth factor (VEGF), monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-2, keratinocyte-derived chemokine (KC), tumor necrosis factor (TNF)-alpha, granulocyte macrophage colony-stimulating
factor (GM-CSF) were measured by high-throughput multiplex bead array cytokine assay based on xMAP (multi-analyte profiling beads) technology.
Results: ER significantly decreased levels of IL-6, IL-10, IL-2, IL-12p70, VEGF, and MCP-1 for 24 hrs incubation at the concentrations of 25, 50, and 100 ??mL in LPS-induced RAW 264.7 cells (P < 0.05). But ER did not exert significant effects on production of MIP-2, KC, TNF-慣, and GM-CSF in LPS-induced RAW 264.7 cells.
Conclusions: These results suggest that ER has an anti-inflammatory property related with its inhibition of cytokine
production in LPS-induced macrophages. |
Keywords:
Red ginseng | Ejung-tang | macrophage | inflammation | cytokine |
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