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JKM > Volume 36(1); 2015 > Article
Lee, Yun, and Yim: The Effect of Gamisoyo-san Intravenous Pharmacopuncture on Restoration of Liver Function after Partial Hepatectomy in SD Rat



This study researched the effect of Gamisoyo-san intravenous pharmacopuncture on restoration of liver function after partial hepatectomy in SD rat.


Twelve rats were randomly divided into three groups: control group (n=4) underwent partial hepatectomy, saline group (n=4) was injected 1ml saline after partial hepatectomy, Gamisoyo-san group (n=4) was injected with 10mg/kg Gamisoyo-san after partial hepatectomy. Liver function and liver regeneration ratio were measured on the seventh day after partial hepatectomy.


The results show that the AST, AST and ALT level in Gamisoyo-san group was significantly lower than those of control group and saline group (P<0.05)


In partial hepatectomy model, Gamisoyo-san intravenous pharmacopuncture seems to significantly promote the restoration of liver function.

Fig. 1.
Serum AST in control group, saline group and Gamisoyo-san group 7 days after partial hepatectomy.
Each value and vertical bar represent the mean±S.D. *P<0.05, vs control group by Mann-Whitney Test, P<0.05, vs saline group by Mann-Whitney Test
Fig. 2.
Serum ALT in control group, saline group and Gamisoyo-san group 7 days after partial hepatectomy.
Each value and vertical bar represent the mean±S.D. *P<0.05, vs control group by Mann-Whitney Test, P<0.05, vs saline group by Mann-Whitney Test
Fig. 3.
Serum ALP in control group, saline group and Gamisoyo-san group 7 days after partial hepatectomy.
Each value and vertical bar represent the mean±S.D. *P<0.05, vs control group by Mann-Whitney Test, P<0.05, vs saline group by Mann-Whitney Test
Fig. 4.
Liver regeneration ratio in control group, saline group and Gamisoyo-san group 7 days after partial hepatectomy.
Each value and vertical bar represent the mean±S.D.
Table 1.
Composition of Gamisoyo-san and Amount of Herbs Used
Name of Herbs Scientific Name Original amount (g) Amount used (g)
목단피 Paeonia suffruticosa 6g 2.25g
백출 Atractylodes japonica 6g 2.25g
적작약 Paeonia lactiflora 4g 1.5g
당귀 Angelica gigas 4g 1.5g
도인 Prunus persica 4g 1.5g
패모 Fritillaria ussuriensis 4g 1.5g
치자 Gardenia jasminoides 3.2g 1.2g
황금 Scutellaria baicalensis 3.2g 1.2g
길경 Platycodon grandiflorum 2.8g 1.05g
청피 Citrus unshiu 2g 0.75g
감초 Glycyrrhiza uralensis 1.2g 0.45g

Sum 40.4g 15.15g
Table 2.
Serum AST, ALT, ALP 7 Days after Partial Hepatectomy Values Represent Mean±S.D. (n=4)
Control group Saline group Gamisoyo-san group
AST 172.35±88.16 227.52±139.02 81.32±9.23
ALT 73.2±32.65 48.65±6.64 31.75±10.35
ALP 1228.4±172.83 1102.02±251.43 741.75±74.66
Table 3.
Liver Regeneration Ratio in Control Group and Saline Group, Gamisoyo–San Group 7 Days After Partial Hepatectomy
Group resected liver (g) regenerated liver (g) liver regeneration ratio (%)
control group 3.84±0.41 11.46±0.82 82
saline group 3.82±0.51 9.99±2.10 71.5
Gamisoyo-san Group 3.89±0.65 12.27±1.06 87.8

Values represent mean ± S.D. (n=4)


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