| 漆皮가 급성림프구성백혈병 세포주 MOLT-4의 세포자멸과 자가포식에 미치는 영향 |
이주영1,2 
, 이장훈1
, 김영철1
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1경희대학교 한의과대학 간계내과학교실
2경희대학교 대학원 임상한의학과 |
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| The Effect of Rhus verniciflua Stokes Extract on Apoptosis and Autophagy in human leukemic cell line (MOLT-4) |
| Juyoung Lee1,2
, Jang-Hoon Lee1
, and Youngchul Kim1
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1Dept. of Internal Medicine, College of Korean Medicine, Kyung Hee University
2Dept. of Clinical Korean Medicine Graduate School, Kyung Hee University |
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| Corresponding Author:
Youngchul Kim ,Tel: 02-958-9118, Fax: 02-958-9258, Email: yckim@khmc.or.kr |
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Received: July 24, 2024; Revised: August 16, 2024. Accepted: August 19, 2024. |
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| ABSTRACT |
Objectives: This study was performed to investigate the effects of Rhus verniciflua Stokes (RVS) on apoptosis and autophagy in the human leukemic cell line, MOLT-4.
Methods: Cell viability was measured by MTS/PMS assay, and cell cycle distribution was analyzed by flow cytometry. The expression levels of mRNA implicated in apoptosis and ER-stress were investigated with RT-qPCR. Lastly, apoptosis- and autophagy-related protein expressions were measured by Western blot analysis.
Results: RVS inhibited proliferation of MOLT-4 in a dose-dependent manner over 24, 48 and 72 hours. RVS treatment also induced an increase in subG1 phase. Exposure to RVS increased the expression of the mRNAs encoding Bax and caspase-3, while decreasing the expression of Bcl-2 mRNA, suggesting that RVS induced apoptosis in MOLT-4 cells. Additionally, RVS extract up-regulated ER-stress related mRNAs such as IRE1α, CHOP, PERK and ATF6. Changes in RVS extract-induced apoptosis and autophagy proteins on MOLT-4 cells were also investigated. The level of Bcl-2 was decreased, whereas the levels of Bax, caspase-3, AMPK, Beclin-1, Atg5, p62, and LC3II were increased.
Conclusion: These results suggest that RVS would be beneficial in the treatment of Acute Lymphoblastic Leukemia. |
| Keywords:
Rhus verniciflua Stokes | Rhois Vernicifluae Cortex | MOLT-4 | Acute Lymphoblastic Leukemia | Apoptosis | Autophagy |
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