Acute toxicity and genotoxicity assessment of <i>Ligigeopoong-san</i>

Objectives: This study aimed to investigate whether Ligigeopoong-san induces acute toxicity and genotoxicity
Methods: Ligigeopoong-san contains the rhizome of Cnidium officinale MAKINO, an herb with potential teratogenicity. Teratogenicity is closely associated with genotoxicity. We analyzed whether Ligigeopoong-san induces acute toxicity and genotoxicity using various experimental models in accordance with Korean non-clinical test standards for pharmaceuticals and OECD test guidelines.
Results: : When Ligigeopoong-san was administered as a single dose to male and female rats, no toxic reactions, including organ damage, were observed at doses up to 2,500 mg/kg. In the bacterial reverse mutation test, no DNA mutations were detected at concentrations up to 5,000 μg/plate. In cell models, Ligigeopoong-san did not induce structural or numerical chromosomal aberrations at concentrations up to 2,000 μg/mL. Additionally, in animal studies, it did not cause bone marrow toxicity or form micronuclei in erythrocytes at doses up to 2,000 mg/kg.
Conclusions: : The experiments using various models demonstrated that Ligigeopoong-san did not induce acute toxicity or genotoxicity.

Keywords: Ligigeopoong-san | Acute toxicity | genotoxicity | Korean medicine | safety