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JKM > Volume 41(2); 2020 > Article
Ha, Choi, Seo, Lee, and Shin: Establishment of the Expiration Date of Herbal Formula Decoction Pouch by Antimicrobial Activities Against Microorganisms

Abstract

Objectives

To establish the expiration date of herbal decoction pouch, the change in antimicrobial activities was measured according to storage temperature and period.

Methods

Five herbal formulas, Gyeji-tang, Jakyakgamcho-tang, Saengmaek-san, Paljung-san, and Dangguisu-san, with antimicrobial activity were selected from 51 herbal formulas commonly used in traditional Korean medical institutions. Five herbal formula decoction pouches were prepared and stored at room temperature (RT) or 4°C from 0 to 12 weeks to evaluate the antimicrobial activity against Bacillus cereus, Listeria monocytogenes, or Vibrio parahaemolyticus. The antimicrobial activities were evaluated by the disk diffusion method (disk size: 10 mm).

Results

The decoction pouches of Gyeji-tang and Jakyakgamcho-tang maintained antimicrobial activity against V. parahaemolyticus from week 0 (14 mm) to week 12 (14 mm) at both temperatures. Saengmaek-san pouch had antimicrobial activities against V. parahaemolyticus and B. cereus from week 0 (19 mm and 20 mm) to week 12 (both 18 mm), respectively. At RT and 4°C, the antimicrobial activity of Paljung-san pouch was maintained week 0 (17 mm) to week 12 (both 13 mm) against B. cereus. Against L. monocytogenes, the antimicrobial activity of Dangguisu-san pouch showed 27 mm at week 0 and 23 mm (RT) and 24 mm (4°C) at week 12, respectively.

Conclusion

According to increase of the storage duration, the antimicrobial activities of 5 herbal formula decoction pouches decreased slightly but remained up to 12 weeks. These results can be used as important data to establish the expiration date of herbal formula decoction pouches.

Fig. 1
Changes of antimicrobial activity against Vibrio parahaemolyticus according to storage temperature and duration of decoction pouch of Gyeji-tang (A), Jakyakgamcho -tang (B), and Saengmaek-san (C). RT: room temperature
jkm-41-2-1f1.gif
Fig. 2
Changes of antimicrobial activity against Bacillus cereus according to storage temperature and duration of decoction pouch of Saengmaek-san (A) and Paljung-san (B). RT: room temperature
jkm-41-2-1f2.gif
Fig. 3
Changes of antimicrobial activity against Listeria monocytogenes according to storage temperature and duration of decoction pouch of Dangguisu-san. RT: room temperature
jkm-41-2-1f3.gif
Table 1
The contents (g) of herbal medicines in herbal formulas
생약명 Latin name Scientific name Gyeji-tang Jakyakgamcho-tang Saengmaek-san Paljung-san Dangguisu-san
계지 (桂枝) Cinnamomi Ramulus Cinnamomum cassia Presl 1500.0
작약 (芍藥) Paeoniae Radix Paeonia lactiflora Pallas 1000.0 1333.3 246.9
감초 (甘草) Glycyrrhizae Radix et Rhizoma Glycyrrhiza uralensis Fischer 500.0 666.7 444.4 123.5
생강 (生薑) Zingiberis Rhizoma Recens Zingiber officinale Roscoe 500.0
대추 (大棗) Zizyphi Fructus Zizyphus jujuba Miller var. inermis Rehder 500.0
맥문동 (麥門冬) Liriopis seu Ophiopogonis Tuber Liriope platyphylla Wang et Tang 1000.0
인삼 (人蔘) Ginseng Radix Panax ginseng C. A. Meyer 500.0
오미자 (五味子) Schisandrae Fructus Schisandra chinensis (Turcz.) Baillon 500.0
대황 (大黃) Rhei Radix et Rhizoma Rheum palmatum Linné 444.4
목통 (木通) Akebiae Caulis Akebia quinata Decaisne 444.4
구맥 (瞿麥) Dianthi Herba Dianthus superbus var. longicalycinus Williams 444.4
편축 (萹蓄) Polygoni Avicularis Herba Polygonum aviculare Linné 444.4
활석 (滑石) Talcum 444.4
치자 (梔子) Gardeniae Fructus Gardenia jasminoides Ellis 444.4
차전자 (車前子) Plantaginis Semen Plantago asiatica Linné 444.4
등심초 (燈心草) Junci Medulla Juncus effusus Linné 444.4
당귀 (當歸) Angelicae Gigantis Radix Angelica gigas Nakai 370.4
오약 (烏藥) Linderae Radix Lindera strichnifolia Fernandez-Villar 246.9
향부자 (香附子) Cyperi Rhizoma Cyperus rotundus Linné 246.9
소목 (蘇木) Sappan Lignum Caesalpinia sappan Linné 246.9
홍화 (紅花) Carthami Flos Carthamus tinctorius Linné 197.5
도인 (桃仁) Persicae Semen Prunus persica Batsch 172.8
육계 (肉桂) Cinnamomi Cortex Cinnamomum cassia Presl 148.1

Total (g) 4000 2000 2000 4000 2000

참고문헌

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20 Seo CS, Shin HK, Kim JH, Shin KS. Changes of principal components and microbial population in Pyungwi-san decoction according to the preservation temperature and period. J Korean Med. 2011; 32:5. 41–9.


21 Kil GJ, Lim DB, Lee YJ. A study on the degraded effect of decocted Yeonkyopaedogsan over a period. J of Herbology. 1998; 13:1. 173–186.


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