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JKM > Volume 39(4); 2018 > Article
ORIGINAL ARTICLE
J Korean Med. 2018;39(4): 40-50.         doi: https://doi.org/10.13048/jkm.18033
Kyungheechunggan-tang suppresses inflammatory cytokines and fibrotic genes in LPS-induced RAW 264.7 cells and LX-2 cells
Junghan Bae  , Eungyeong Jang  , and Jang-Hoon Lee 
Department of Internal Medicine, College of Korean Medicine, Kyung Hee University
Corresponding Author: Jang-Hoon Lee ,Tel: +82-2-958-9118, Fax: +82-2-958-9258, Email: komclive@khmc.or.kr
Received: April 16, 2018;  Revised: May 8, 2018.  Accepted: May 8, 2018.
ABSTRACT
Objectives: The aim of this study is to investigate anti-inflammatory effects of Kyungheechunggan-tang (KHCGT) on LPS-induced RAW 264.7 cells and LX-2 cells and anti-fibrotic effects of KHCGT on LX-2 cells. Materials and Methods: Three types of KHCGTs (KHCGT-A, -B, and -C) by narrowing down the number of constituent herbs from 9 (KHCGT-A) to 5 (KHCGT-B) and to 3 (KHCGT-C) were developed. To understand pharmacological effects of KHCGT, three types of KHCGTs were treated on RAW 264.7 cells and LX-2 cells. Anti-inflammatory activities of KHCGT were evaluated by ELISA assay for pro-inflammatory cytokines, IL-6, TNF- α and IL-10, in LPS-stimulated RAW 264.7 cells and for IL-6 production in LPS-induced LX-2 cells. In addition, anti-fibrotic effects of KHCGT were determined by quantitative real-time PCR assay for fibrosis-related genes, α-SMA, collagen1A1, TIMP1, MMP-2, in LX-2 cells.
Results: KHCGT-A and KHCGT-C showed inhibitory effects on secretion of IL-6 in LPS-stimulated RAW 264.7 cells and LX-2 cells. KHCGT-B and KHCGT-C exhibited inhibitory effects on the expression of pro-inflammatory cytokines such as IL-6, TNF- α, and IL-10 in LPS-stimulated RAW 264.7 cells. The mRNA expression levels of collagen1A1 and MMP-2 were significantly reduced by KHCGT-C whereas TIMP-1 was suppressed by KHCGT-A and KHCGT-B in LX-2 cells. Among three different formulas, KHCGT-C demonstrated the most remarkable effects on inflammation and fibrosis.
Conclusions: In this study, KHCGT showed both anti-inflammatory and anti-fibrotic effects which make it to be a prospective agent for chronic liver diseases with inflammation and fibrosis.
Keywords: Kyungheechunggan-tang | RAW 264.7 | LX-2 | Inflammation | Fibrosis
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