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JKM > Volume 39(4); 2018 > Article
ORIGINAL ARTICLE
J Korean Med. 2018;39(4): 86-113.         doi: https://doi.org/10.13048/jkm.18037
Anti-osteoarthritic effects of a combination of pomegranate concentrate powder, Eucommiae cortex and Achyranthis radix in rats
Beom-Rak Choi1,2, Sae-Kwang Ku3,4  , Su-Jin Kang4,5, Hye-Rim Park1, Mi-Sun Sung1, Young-Joon Lee4,5  , and Ki-Moon Park2
1Research Institute, HL Science Co., Ltd., Republic of Korea
2Department of Foodscience and biotechnology, Sungkyunkwan University, Republic of Korea
3Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University, Republic of Korea
4The Medical Research Center for Globalization of Herbal Medicine, Daegu Haany University, Republic of Korea
5Department of Preventive Medicine, College of Korean Medicine, Daegu Haany University, Republic of Korea
Corresponding Author: Young-Joon Lee ,Tel: +82-53-819-1296, Fax: +82-53-819-1576, Email: gksxntk@dhu.ac.kr
Corresponding Author: Ki-Moon Park ,Tel: +82-31-290-7806, Email: Pkm1001@skku.edu
Received: August 16, 2018;  Revised: December 12, 2018.  Accepted: December 12, 2018.
ABSTRACT
Objectives: We examined the effects of a mixed formula consisting of dried pomegranate concentrate powder (PCP) and the aqueous extracts of Eucommiae cortex (EC) and Achyranthis radix (AR) in rats with surgically induced osteoarthritis (OA).
Methods: Two weeks after OA-inducing surgery, a PCP:EC:AR 5:4:1 (g/g) combination or single formula was orally administered. Changes in body weight, knee thickness, maximum knee extension angle, bone mineral density of the knee joints, femoral and tibial articular surfaces, and compressive strength of the femoral and tibial articular cartilage (AC) were assessed, along with the prostaglandin E2 level, 5-lipoxygenase, matrix metalloproteinase (MMP)-2 and MMP-9 activity, and chondrogenic gene mRNA expression in the femoral and tibial AC with the synovial membrane (SM). In addition, the number of cleaved poly(ADP-ribose) polymerase, cyclooxygenase and tumor necrosis factor-α-immunoreactive cells in the femoral and tibial AC with SM were monitored, and the rate of cell proliferation was determined with a 5-bromo-2’-deoxyuridine uptake assay. Results : The signs of surgically induced OA in rats were significantly inhibited by both PCP, EC and AR combined and single formulas. In particular, the combination formula-treated OA model rats showed dose-dependent, significantly increased inhibitory activity against all tested criteria compared with single formula-treated rats.
Conclusions: Taken together, our results suggest that the combination formula synergistically increased the anti-OA effects of its components through anti-inflammatory and chondrogenic activity in rats with surgically induced OA. In addition, 200, 100 and 50 mg/kg combination formula treatments showed dose-dependent inhibitory activity against all of the tested criteria.
Keywords: Dried pomegranate concentrate powder | Eucommiae Cortex | Achyranthis Radix | PCP:EC:AR 5:4:1 (g/g) mixed formulation | Efficacy confirmation | Surgically induced osteoarthritic rats
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