A Study of the Effect on Obesity and dyslipidemia in Kidney-hypofunction Animal Model Induced by Unilateral Ureteral Obstruction

Jinyoung Kwak; Junghwan Park; Youngmee Koh; Taekwon Ahn; Kwak, Jinyoung; Park, Junghwan; Koh, Youngmee; Ahn, Taekwon

doi:10.13048/jkm.18011

JKM > Volume 39(2); 2018 > Article

Kwak, Park, Koh, and Ahn: A Study of the Effect on Obesity and dyslipidemia in Kidney-hypofunction Animal Model Induced by Unilateral Ureteral Obstruction

Original Article

The Journal of Korean Medicine 2018; 39(2): 1-12.

Published online: June 30, 2018

DOI: https://doi.org/10.13048/jkm.18011

A Study of the Effect on Obesity and dyslipidemia in Kidney-hypofunction Animal Model Induced by Unilateral Ureteral Obstruction

Jinyoung Kwak¹, Junghwan Park¹, Youngmee Koh¹, Taekwon Ahn¹

¹Dept. of Sasang Constitutional Medicine, College of Korean Medicine, Daejeon University

Correspondence to: 안택원(Taekwon Ahn) Dept. of Sasang Constitutional Medicine, Daejeon Univ. Cheonan Hospital of Korean Medicine. Notaesan-ro 4, Seobuk-gu, Cheonan city, Chungcheongnamdo, 31099(Zip code), Korea. Tel: +82-41-521-7535, Fax: +82-41-521-7007, E-mail: twahn@dju.kr

Received March 15, 2018 Revised May 5, 2018 Accepted June 4, 2018

Abstract

Objectives

The objective of this study is to develop a new animal model with Kidney-hypofunction for Sasang Constitutional Medicine, especially for partial Soyangin(one of four constitution which has good digestive function and poor renal function) by Unilateral Ureteral Obstruction, and to estimate the factor related to obesity, dyslipidemia, and metabolic syndrome.

Methods

The C57BL/6J mice were divided into 3 groups: normal group, high fat diet(HFD) control group, and HFD group with Unilateral Ureteral Obstruction(UUO). Then, the HFD control group and the experimental group were fed with high fat diet for 6 weeks. Food intake and body weight were measured at regular time by week. After the final experiment, blood was gathered for bloodchemical examination and organs(liver, fatty tissue) were remoed, weighted, and mRNA was analyzed with real-time PCR.

Results

The weight growth rate with High fat diet went down by 8.35% in experimental group and had similar FER with the normal group, while HFD control group had higher weight growth rate and FER than any other groups. Also The experimental group had lower triglyceride and LDL cholesterol rate and higher glucose rate in serum. and in mRNA expression, GLUT-9, the protein related to excretion of uric acid and metabolic syndrome, expressed lower rate than that of HFD control group. and IL-6, a kind of cytokine related to obesity and metabolic syndrome, expressed more than HFD control group.

Conclusions

It was found that Kidney-hypofunction animal-experimental model is susceptible to metabolic syndrome.

Keywords: Obesity, Soyangin, Animal-Experimental Model, High-fat Diet

Fig. 1

radical nephrectomy and UUO establishment

Fig. 2

Body weight change in HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.

C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5)

UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)

Fig. 3

Food efficiency ratio (FER, %) in HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.

C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5)

UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)

Fig. 4

AST, ALT level in serum of HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.

C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5)

UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)

Fig. 5

Creatine level in serum of HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.

C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5) UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)

Fig. 6

Total Cholesterol level in serum of HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.

C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5) UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)

Fig. 7

HDL and LDL Cholesterol level in serum of HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.

C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5) UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)

Fig. 8

Triglyceride level in serum of HFD-induced

Fig. 9

Glucose level in serum of HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.

C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5) UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)

Fig. 10

Abdominal subcutaneous fat and Epididymal adipose tissue weight in HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.

C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5) UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)

Fig. 11

Liver weight in HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.

C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5) UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)

Fig. 12

mRNA expression of GLUT-9, IL-6, COX-2 were measured with Real Time-PCR analysis.

C57bl/6J: C57bl/6J normal group (n=5)

HFD-CTL: High Fat Diet-Negative Control (n=5)

UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)

Table 1

Food Intake, Body Weight gain, Food Efficiency Ratio in HFD-Fed Obese Mice, and -Renal Cortex of Each Study Group(UUO: Unilateral Ureteral Obstruction) Obese Mice.

Group	Food intakes	Bodyweight gain	Food Efficiency

	(g/day)	(g/day)	(FER, %)
C57bl/6-Nr	3.46	0.191±0.01	5.53±0.31
HFD-CTD	2.89	0.377±0.03	13.05±0.92
UUO_HFD-CTD	5.97	0.280±0.03	4.70±1.29

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