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JKM > Volume 37(1); 2016 > Article
Lee, Choi, and Yim: The Effect of Jeokbaekhaogwanjung-tang Herbal Acupuncture Solution on the Immune Activity of Spleen Cells of Aged Rats

Abstract

Objectives:

The purpose of this study was to investigate the effect of Jeokbaekhaogwanjung-tang (JGT) distillate on the immune activity of spleen cells of aged rats.

Methods:

Spleen cells of 10w, 52w 72w old SD rats were cultured and treated with JGT distillate and the levels of IL-2, IL-4, IL-10 and IFN-γ were measured.

Results:

  1. The levels of IL-2 and IFN-γ in the spleen cells of 52w old rats were significantly decreased by JGT treatment.

  2. The level of IFN-γ in the spleen cells of 72w old rats were significantly decreased by JGT treatment.

Conclusion:

These results suggest that Jeokbaekhaogwanjung-tang distillate has an immune regulative effect by way of suppressing the change of immune activity caused by aging in rats.

Fig. 1.
Effect of Aging on the levels of various immune cytokines in the spleen cell culture.
The levels of various immune cytokines in rat’s spleen cell culture were analysed by ELISA. Values represent mean ± SD (n=5) NT : Cells with no treatment. ConA : Cells treated with ConA for 24 hours.
Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours.
***: p<0.001, **: p<0.01 compared to 10w group. †: p<0.05 compared to 52w group.
jkm-37-1-90f1.tif
Fig. 2.
Effect of JGT distillate on IL-2 level in spleen cell culture of aged rat
The level of IL-2 in rat’s spleen cell culture was analysed by ELISA. Values represent mean ± SD (n=5)
NT : Cells with no treatment. ConA : Cells treated with ConA for 24 hours.
Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours.
***: p<0.001, **: p<0.01 compared to NT group.
†: p<0.05 compared to ConA group. ‡: p<0.05 compared to Vit.C group.
jkm-37-1-90f2.tif
Fig. 3.
Effect of JGT distillate on IL-4 level in spleen cell culture of aged rat
The level of IL-4 in rat’s spleen cell culture was analysed by ELISA. Values represent mean ± SD (n=5)
NT : Cells with no treatment. ConA : Cells treated with ConA for 24 hours.
Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours.
***: p<0.001, *: p<0.05 compared to NT group.
††† : p<0.001, †† : p<0.01 compared to ConA group. ‡: p<0.01 compared to Vit.C group.
jkm-37-1-90f3.tif
Fig. 4.
Effect of JGT distillate on IL-10 level in spleen cell culture of aged rat
The level of IL-10 in rat’s spleen cell culture was analysed by ELISA. Values represent mean ± SD (n=5)
NT : Cells with no treatment. ConA : Cells treated with ConA for 24 hours.
Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours.
***: p<0.001, **: p<0.01 compared to NT group.
††† : p<0.001 ††: p<0.01 compared to ConA group. ‡‡: p<0.01, ‡: p<0.05 compared to Vit.C group.
jkm-37-1-90f4.tif
Fig. 5.
Effect of JGT distillate on IFN-γ level in spleen cell culture of aged rat
The level of IFN-γ in rat’s spleen cell culture was analysed by ELISA. Values represent mean ± SD (n=5)
NT : Cells with no treatment. ConA : Cells treated with ConA for 24 hours.
Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours. *** : p<0.001 compared to NT group.
†††: p<0.001 : p<0.05 compared to ConA group. ‡‡: p<0.01 compared to Vit.C group.
jkm-37-1-90f5.tif
Table 1.
The Compositions of Jeokbaekhaogwanjung-tang(JGT)
Herbs Pharmacognostic name Dose(g)
白何首烏 Cynanchi Wilfordii Radix 4
赤何首烏 Polygoni Multiflori Radix 4
良薑 Alpiniae Officinari Rhizoma 4
乾薑 Zingiberis Rhizoma 4
陳皮 Citri Unshii Pericarpium 4
靑皮 Citrii Unshiu Immaturi Pericarpium 4
香附子 Cyperi Rhizoma 4
益智仁 Aipiniae Fructus 4
棗二 Zizyphi Fructus 7

Total 39

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