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JKM > Volume 36(1); 2015 > Article
Lee, Han, and Yang: The effects of Atractylodes japonica Koidz. on type 2 diabetic rats



Type 2 diabetes mellitus is a metabolic disease characterized by insulin resistance and high blood glucose level from progressive insulin secretory defect. The rhizome of Atractylodes japonica Koidz. (AJ) has been used for treatment of retention of water in oriental medicine. The aim of this study is to examine the effects of AJ on type 2 diabetes rats.


Type 2 diabetes was induced by 60% high fat diet and low dose streptozotocin. Rats were divided into 4 groups (n = 6); Nor (normal control group), Con (diabetic group treated with vehicle), Met (diabetic group treated with 200 mg/kg metformin) and AJ (diabetic group treated with 100 mg/kg AJ). The body weights and food intakes were measured during the treatment period. After 4 weeks treatment, blood glucose level, HOMA-IR, and protein expressions of IRS-1, p-IRS-1, PPAR-γ, and GLUT4 were measured, and histopathological examination of beta cell was performed.


Compared with the control group, blood glucose level and HOMA-IR were reduced in rats treated with AJ. Impaired beta cells in pancreas of rats were recovered and phosphorylation of IRS-1 was increased in rats treated with AJ. And also, protein expressions of PPAR-γ and GLUT4 were increased by treatment of AJ.


The results suggest that Atractylodes japonica Koidz. may have anti-diabetic effect on type 2 diabetic rats through regulation of blood glucose level and insulin resistance. Therefore Atractylodes japonica Koidz. may have positive effects on patients with type 2 diabetes.

Fig. 1.
Time change on body weight, food intake in diabetic rats. Each value is expressed as mean ± S.E.M. (n = 6). a, p<0.05 when compared to the normal control. b, p<0.05 when compared to the negative control.
Fig. 2.
Blood glucose level in diabetic rats. Each value is expressed as mean ± S.E.M. (n = 6). a, p<0.05 when compared to the normal control. b, p<0.05 when compared to the negative control.
Fig. 3.
Homeostatic model assessment-insulin resistance (HOMA-IR) in diabetic rats. The results were calculated with following formula: fasting blood glucose (mmol/L) × insulin (µU/mL) / 22.5. Each value is expressed as mean ± S.E.M. (n = 6).
Fig. 4.
Histological examination of β-islet in rat pancreas. (A) normal rats, (B) diabetic rats treated with vehicle, (C) diabetic rats treated with metformin, (D) diabetic rats treated with AJ. The magnification was × 400.
Fig. 5.
Effects of AJ on IRS-1 and p-IRS-1 in the liver of diabetic rats. The expression of IRS-1 and phospholyrated IRS-1 were determined by western blot analysis. a, p<0.05 when compared to the normal control. b, p<0.05 when compared to the negative control.
Fig. 6.
Effects of AJ on GLUT4 and PPAR-γ in the skeletal muscle of diabetic rats. The expression of PPAR-γ and GLUT4 were determined by western blot analysis. a, p<0.05 when compared to the normal control. b, p<0.05 when compared to the negative control.


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