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JKM > Volume 36(1); 2015 > Article
Lee, Han, and Yang: The effects of Atractylodes japonica Koidz. on type 2 diabetic rats

Abstract

Objectives:

Type 2 diabetes mellitus is a metabolic disease characterized by insulin resistance and high blood glucose level from progressive insulin secretory defect. The rhizome of Atractylodes japonica Koidz. (AJ) has been used for treatment of retention of water in oriental medicine. The aim of this study is to examine the effects of AJ on type 2 diabetes rats.

Methods:

Type 2 diabetes was induced by 60% high fat diet and low dose streptozotocin. Rats were divided into 4 groups (n = 6); Nor (normal control group), Con (diabetic group treated with vehicle), Met (diabetic group treated with 200 mg/kg metformin) and AJ (diabetic group treated with 100 mg/kg AJ). The body weights and food intakes were measured during the treatment period. After 4 weeks treatment, blood glucose level, HOMA-IR, and protein expressions of IRS-1, p-IRS-1, PPAR-γ, and GLUT4 were measured, and histopathological examination of beta cell was performed.

Results:

Compared with the control group, blood glucose level and HOMA-IR were reduced in rats treated with AJ. Impaired beta cells in pancreas of rats were recovered and phosphorylation of IRS-1 was increased in rats treated with AJ. And also, protein expressions of PPAR-γ and GLUT4 were increased by treatment of AJ.

Conclusions:

The results suggest that Atractylodes japonica Koidz. may have anti-diabetic effect on type 2 diabetic rats through regulation of blood glucose level and insulin resistance. Therefore Atractylodes japonica Koidz. may have positive effects on patients with type 2 diabetes.

Fig. 1.
Time change on body weight, food intake in diabetic rats. Each value is expressed as mean ± S.E.M. (n = 6). a, p<0.05 when compared to the normal control. b, p<0.05 when compared to the negative control.
jkm-36-1-75f1.tif
Fig. 2.
Blood glucose level in diabetic rats. Each value is expressed as mean ± S.E.M. (n = 6). a, p<0.05 when compared to the normal control. b, p<0.05 when compared to the negative control.
jkm-36-1-75f2.tif
Fig. 3.
Homeostatic model assessment-insulin resistance (HOMA-IR) in diabetic rats. The results were calculated with following formula: fasting blood glucose (mmol/L) × insulin (µU/mL) / 22.5. Each value is expressed as mean ± S.E.M. (n = 6).
jkm-36-1-75f3.tif
Fig. 4.
Histological examination of β-islet in rat pancreas. (A) normal rats, (B) diabetic rats treated with vehicle, (C) diabetic rats treated with metformin, (D) diabetic rats treated with AJ. The magnification was × 400.
jkm-36-1-75f4.tif
Fig. 5.
Effects of AJ on IRS-1 and p-IRS-1 in the liver of diabetic rats. The expression of IRS-1 and phospholyrated IRS-1 were determined by western blot analysis. a, p<0.05 when compared to the normal control. b, p<0.05 when compared to the negative control.
jkm-36-1-75f5.tif
Fig. 6.
Effects of AJ on GLUT4 and PPAR-γ in the skeletal muscle of diabetic rats. The expression of PPAR-γ and GLUT4 were determined by western blot analysis. a, p<0.05 when compared to the normal control. b, p<0.05 when compared to the negative control.
jkm-36-1-75f6.tif

참고문헌

1. Mccarthy MI. Genomics, type 2 diabetes, and obesity. The New England Journal of Medicine. 2010; 363:24. 2339–2350.
crossref

2. Kahn SE, Hull RL, Utzschneider KM. Mechanisms linking obesity to insulin resistance and type 2 diabetes. Nature. 2006; 444:7121. 840–846.
crossref

3. Korean diabetes association. Korea centers for disease control and prevention. Diabetes fact sheet in korea 2012.


4. Alberti KGMM, Zimmet PZ. Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus. Provisional report of a WHO Consultation. Diabetic medicine: a journal of the British Diabetic Association. 1998; 15:7. 539–553.
crossref

5. American diabetes association. Standards of medical care in diabetes-2014. Diabetes Care. 2014; 37:1. S14–S80.
crossref

6. Cheng AYY, Fantus IG. Oral antihyperglycemic therapy for type 2 diabetes mellitus. Canadian Medical Association journal. 2005; 172:2. 213–226.
crossref

7. Yale JF. Oral antihyperglycemic agents and renal disease: New agents, new concepts. Journal of the American Society of Nephrology. 2005; 16:S7–S10.
crossref

8. Doo HK. Internal Medicine of Kidney System. Seongbosa. 2003; 1131


9. Kang SB, Kim GC. A pathological approach about symptoms of So-gal. The Journal of East-West Medicines. 1998; 23:4. 21–40.


10. Kang SB. The comparative study between the transformations of sogal and the complications of diabetes mellitus. The Journal of Korean Oriental Medical Society. 1998; 19:2. 137–152.


11. Editing commission of herbal medicine. Herbal medicine. Youngrimsa. 2007; 331–332.


12. Ahn DK. Illustrated guide to korean herbal medicine. Kyohaksa. 2008; 384


13. Heo J. Donguibogam. Bubinmunhwasa. 2007; 1952


14. Choi KH, Jeong SI, Lee JH, Hwang BS, Kim SJ, Lee S, et al. Pharmacological mechanism responsible for the Atractylodes japonica-induced distal colonic contraction in rats. Phytomedicine. 2011; 18:408–413.
crossref

15. Han YK, Jung HW, Park YK. The roots of Atractylodes japonica Koidzumi promote adipogenic differentiation via activation of the insulin signalong pathway in 3T3-L1 cells. BMC Complementary and Alternative Medicine. 2012; 12:154
crossref

16. Han HK, Yoon SJ, Kim GH. Effects of compositae plants on plasma glucose and lipid level in streptozotocin induced diabetic rats. Journal of Korean Food Science and Nutrition. 2009; 38:6. 674–682.
crossref

17. Chen F, Xiong H, Wang J, Ding X, Shu G, Mei Z. Antidiabetic effect of total flavonoids from Sanguis draxonis in type 2 diabetic rats. Journal of ethnopharmacology. 2013; 149:3. 729–736.
crossref

18. Vogeser M, König D, Frey I, Predel HG, Parhofer KG, Berg A. Fasting serum insulin and the homeostasis model of insulin resistance (HOMA-IR) in the monitoring of lifestyle interventions in obese persons. Clinical Biochemistry. 2007; 40:13–14. 964–968.
crossref

19. Catalano PM, Presley L, Minium J, Mouzon SHD. Fetuses of obese mothers develop insulin resistance in utero. Diabetes Care. 2009; 32:40. 1076–1080.
crossref

20. Han YK, Park YK. Effect of Atractylodis Rhizoma Alba water extract on streptozotocin-induced diabetes in rats. Journal of Korean Herbology. 2011; 26:4. 23–30.


21. Nolan CJ, Damm P, Prentki M. Type 2 diabetes across generations: from pathophysiology to prevention and management. The Lancet. 2011; 378:9. 169–181.
crossref

22. Adefegha SA, Oboh G, Adefegha OM, Boligon AA, Athayde ML. Antihyperglycemic, hypolipidemic, hepatoprotective and antioxidative effects of dietary clove (Szyzgium aromaticum) bud powder in a high-fat diet/streptozotocin-induced diabetes rat model. Journal of the science of food and agriculture. 2014; 94:13. 2726–2737.
crossref

23. Colberg SR, Albright AL, Blissmer BJ, Braun B, Chasan-Taber L, Fernhall B, et al. Exercise and Type 2 Diabetes. Medicine and Science in Sports and Exercise. 2010; 42:12. 2282–2303.
crossref

24. Hong SM, Park JH, Lim YH, Park YS, Kim DS, Choi WH, et al. The relationship between diabetic retinopathy and macrovascular complication in patients with type 2 diabetes. The Korean Journal of Medicine. 2011; 81:3. 351–358.


25. Chae HJ, Lee IS, Moon HY. Effects of Schizandra Cchinensis fruit extract on the hyperglycemia and hyperlipemia in streptozotocin-induced diabetic rats. Korean Society for Biotechnology and Bioengineering Journal. 2011; 26:2. 126–130.
crossref

26. Choi ES, Rhee EJ, Kim JH, Won JC, Park CY, Lee WY, et al. Insulin sensitivity and insulin secretion determined by homeostasis model assessment and future risk of diabetes mellitus in korean men. Diabetes and Metabolism Journal. 2008; 32:6. 498–505.
crossref

27. Han JS, Back SH, Hur JU, Lin YH, Gildersleeve R, Shan J, et al. ER-stress-induced transcriptional regulation increases protein synthesis leading to cell death. Nature cell biology. 2013; 15:5. 481–490.
crossref

28. Dor Y, Glaser B. Beta-cell differentiation and type 2 diabetes. The New England Journal of Medicine. 2013; 368:6. 572–573.
crossref

29. Imamura F, Mukamal KJ, Meigs JB, Luchsinger JA, Ix JH, Siscovick DS, et al. Risk factors for type 2 diabetes mellitus preceded by beta-cell dysfunction, insulin resistance, or both in older adults. American Journal of Epidemiology. 2013; 177:12. 1418–1429.
crossref

30. Ryu HS, Park SY, Ma D, Zhang J, Lee W. The Induction of MicroRNA Targeting IRS-1 Is Involved in the Development of Insulin Resistance under Conditions of Mitochondrial Dysfunction in Hepatocytes. PloS one. 2011; 6:3. e17343
crossref

31. Saltiel AR, Kahn CR. Insulin signalling and the regulation of glucose and lipid metabolism. Nature. 2001; 414:6865. 799–806.
crossref

32. Hemi R, Yochananov Y, Barhod E, Kasher MM, Karasik A, Tirosh A, et al. p38 mitogen-activated protein kinase-dependent transactivation of ErbB receptor family: a novel common mechanism for stress-induced IRS-1 serine phosphorylation and insulin resistance. Diabetes. 2011; 60:4. 1134–1145.
crossref

33. Ohshima K, Mogi M, Jing F, Iwanami J, Tsukuda K, Min LJ, et al. Direct angiotensin II type 2 receptor stimulation ameliarates insulin resistance in type 2 diabetes mice with ppar γ activation. PloS one. 2012; 7:11. e48387


34. Olson AL, Pessin JE. Structure, function, and regulation of the mammalian facilitative glucose transporter gene family. Annual review of nutrition. 1996; 16:235–256.
crossref

35. Cline GW, Petersen KF, Krssak M, Shen J, Hundal RS, Trajanoski Z, et al. Impaired glucose transport as a cause of decreased insulin-stimulated muscle glycogen synthesis in type 2 diabetes. The New England Journal of Medicine. 1999; 341:4. 240–246.
crossref

36. Kainulainen H, Breiner M, Schurmann A, Marttinen A, Virjo A, Joost HG. In vivo glucose uptake and glucose transporter proteins GLUT1 and GLUT4 in heart and various types of skeletal muscle from streptozotocin-diabetic rats. Biochimica et Biophysica Acta. 1994; 1225:3. 275–282.
crossref

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