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JKM > Volume 39(2); 2018 > Article
Song and Baek: A Comparative Study of Sepiae Os, Arcae Concha, Ostreae Concha and Esomeprazole in a Mouse Model of Reflux Esophagitis

Abstract

Objectives

This aim of this study is to compare the reflux esophagitis improvement effects of Sepiae Os, Arcae Concha, Ostreae Concha, and Proton Pump Inhibitor(esomeprazole) through rat experiments.

Methods

NO production inhibitory effect was measured by NO production amount and iNOS mRNA expression level in cell lines. iNOS, TNF-α and p-IκB, and serotonin were compared using immunohistochemistry at the rat reflux esophagitis. Reflux esophagitis connection external form, lower esophageal sphincter, and gap were observed and an esophageal inflammatory indicator, IL-6 activity was also evaluated by immunohistochemistry.

Results

NO production and iNOS mRNA expression was showed concentration dependent decrease in cell lines treated with Sepiae OS, Arcae Concha, and Ostreae Concha at the experiments of cell lines. In the suppression of iNOS and p-IκB at the rat reflux esophagitis, Sepiae Os treat group(SOT) and Ostreae Concha treat group(OCT) were more effective. In the increase of serotonin at the rat reflux esophagitis, ACT, MT and OCT were more effective. Damage of lower esophageal sphincter, and gap between esophageal keratin and mucosa were observed less at the SOT, ACT, OCT. In the suppression of IL-6 at the rat reflux esophagitis, SOT and OCT were more effective than GE and, SOT was more effective than MT significantly.

Conclusions

The anti-inflammatory effect was the best in the SOT and lower esophageal sphincter muscle contraction was the best in the ACT at the rat reflux esophagitis. Sepiae OS was more effective than esomeprazole in the suppression of iNOS, TNF-α, and IL-6.

Fig. 1
Experimental design and reflux esophagitis animal model
Abbreviation. HFD, high fat diet; SO, Sepiae Os; AC, Arcae Concha; OC, Ostreae Concha.
jkm-39-2-92f1.gif
Fig. 2
Inhibition of NO production and iNOS mRNA expression by Sepiae Os (SO)
jkm-39-2-92f2.gif
Fig. 3
Inhibition of NO production and iNOS mRNA expression by Arcae Concha (AC)
jkm-39-2-92f3.gif
Fig. 4
Inhibition of NO production and iNOS mRNA expression by Ostrea Concha (OC)
jkm-39-2-92f4.gif
Fig. 5
The iNOS positive reaction in gastroesophageal junction (GEJ) with reflux esophagitis
Abbreviation. Ctrl, no reflux esophagitis elicited group; GE, reflux esophagitis elicited group; MT, medicine as esomeprazole treat group before reflux esophagitis elicitation; SOT, Sepiae Os (SO) treat group before reflux esophagitis elicitation; ACT, Arcae Concha (AC) treat group before reflux esophagitis elicitation; OCT, Ostreae Concha (OC) treat group before reflux esophagitis elicitation; Bar size, 200μm; arrow, iNOS positive reaction; LES, lower esophageal sphincter; ES, esophagus; LP, lamina propria.
jkm-39-2-92f5.gif
Fig. 6
The TNF-α positive reaction in gastroesophageal junction (GEJ) with reflux esophagitis
jkm-39-2-92f6.gif
Fig. 7
The p-IκB positive reaction in gastroesophageal junction (GEJ) with reflux esophagitis
jkm-39-2-92f7.gif
Fig. 8
The Serotonin positive reaction in gastroesophageal junction (GEJ) with reflux esophagitis
jkm-39-2-92f8.gif
Fig. 9
The improvement effect of hemorrhagic erosion in GEJ by SO, AC, OC and Esomeprazole (a photo ×4; b photo bar size 200μm)
jkm-39-2-92f9.gif
Fig. 10
The IL-6 positive reaction in gastroesophageal junction (GEJ) with reflux esophagitis
jkm-39-2-92f10.gif

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