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JKM > Volume 39(2); 2018 > Article
Kwak, Park, Koh, and Ahn: A Study of the Effect on Obesity and dyslipidemia in Kidney-hypofunction Animal Model Induced by Unilateral Ureteral Obstruction

Abstract

Objectives

The objective of this study is to develop a new animal model with Kidney-hypofunction for Sasang Constitutional Medicine, especially for partial Soyangin(one of four constitution which has good digestive function and poor renal function) by Unilateral Ureteral Obstruction, and to estimate the factor related to obesity, dyslipidemia, and metabolic syndrome.

Methods

The C57BL/6J mice were divided into 3 groups: normal group, high fat diet(HFD) control group, and HFD group with Unilateral Ureteral Obstruction(UUO). Then, the HFD control group and the experimental group were fed with high fat diet for 6 weeks. Food intake and body weight were measured at regular time by week. After the final experiment, blood was gathered for bloodchemical examination and organs(liver, fatty tissue) were remoed, weighted, and mRNA was analyzed with real-time PCR.

Results

The weight growth rate with High fat diet went down by 8.35% in experimental group and had similar FER with the normal group, while HFD control group had higher weight growth rate and FER than any other groups. Also The experimental group had lower triglyceride and LDL cholesterol rate and higher glucose rate in serum. and in mRNA expression, GLUT-9, the protein related to excretion of uric acid and metabolic syndrome, expressed lower rate than that of HFD control group. and IL-6, a kind of cytokine related to obesity and metabolic syndrome, expressed more than HFD control group.

Conclusions

It was found that Kidney-hypofunction animal-experimental model is susceptible to metabolic syndrome.

Fig. 1
radical nephrectomy and UUO establishment
jkm-39-2-1f1.gif
Fig. 2
Body weight change in HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.
C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5)
UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)
jkm-39-2-1f2.gif
Fig. 3
Food efficiency ratio (FER, %) in HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.
C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5)
UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)
jkm-39-2-1f3.gif
Fig. 4
AST, ALT level in serum of HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.
C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5)
UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)
jkm-39-2-1f4.gif
Fig. 5
Creatine level in serum of HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.
C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5) UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)
jkm-39-2-1f5.gif
Fig. 6
Total Cholesterol level in serum of HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.
C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5) UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)
jkm-39-2-1f6.gif
Fig. 7
HDL and LDL Cholesterol level in serum of HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.
C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5) UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)
jkm-39-2-1f7.gif
Fig. 8
Triglyceride level in serum of HFD-induced
jkm-39-2-1f8.gif
Fig. 9
Glucose level in serum of HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.
C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5) UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)
jkm-39-2-1f9.gif
Fig. 10
Abdominal subcutaneous fat and Epididymal adipose tissue weight in HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.
C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5) UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)
jkm-39-2-1f10.gif
Fig. 11
Liver weight in HFD-induced obese mice, and -renal cortex of each study group(UUO: unilateral ureteral obstruction) obese mice.
C57bl/6J-Nr: C57bl/6J normal group (n=5) HFD-CTL: High Fat Diet-Negative Control (n=5) UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)
jkm-39-2-1f11.gif
Fig. 12
mRNA expression of GLUT-9, IL-6, COX-2 were measured with Real Time-PCR analysis.
C57bl/6J: C57bl/6J normal group (n=5)
HFD-CTL: High Fat Diet-Negative Control (n=5)
UUO_HFD-CTL: High Fat Diet group with unilateral ureteral obstruction (n=5)
jkm-39-2-1f12.gif
Table 1
Food Intake, Body Weight gain, Food Efficiency Ratio in HFD-Fed Obese Mice, and -Renal Cortex of Each Study Group(UUO: Unilateral Ureteral Obstruction) Obese Mice.
Group Food intakes Bodyweight gain Food Efficiency

(g/day) (g/day) (FER, %)
C57bl/6-Nr 3.46 0.191±0.01 5.53±0.31
HFD-CTD 2.89 0.377±0.03 13.05±0.92
UUO_HFD-CTD 5.97 0.280±0.03 4.70±1.29

참고문헌

1 Lee JM. Dongeisusebowon. 2nd rev. Seoul: Yeogang;2003. 36:p. 278(Korean).


2 Hwang MW. The lecture of Sasang constitution. 2nd Edition. Seoul: Koonja;2012. p. 33–40. (Korean).


3 Dept of Sasang Constitutional Medicine. All colleges of Korean med in Korea(compilation). The revised and enlarged Sasang Constitutional Medicine. Seoul: Jipmoondang;2012. p. 136–144. (Korean).


4 Hwang MW, Koh BH. The Study on the Pathology of Soyangin in Sasang Constitutional Medicine(SCM). Journal of Sasang Constitutional Medicine. 2009; 21:3. 1–16.


5 Lalich JJ, Burkholder PM, Paik WC. Protein overload nephropathy in rats with unilateral nephrectomy. A correlative light immunogluorescence and electron microscopical analysis. Archives of pathology. 99:2. 1975; 72–79.


6 Park SB. The Relation of Cardiovascular Risk Factors to Metabolic Syndrome. Journal of the Korean Academy of Family Med. 2005; 26:10. 20–26.


7 Sim JY, Kang HT, Kim SY, Kim JS, Kim JW, Kim JY, et al. The Prevention and Treatment of Metabolic Syndrome in Korean Adults. Korean Journal of Family Practice. 2015; 5:3. 375–420.


8 Perk J, De Backer G, Gohlke H, Graham I, Reiner Z, Verschuren M, et al. European Guidelines on cardiovascular disease prevention in clinical practice (version 2012). The Fifth Joint Task Force of the European Society of Cardiology and Other Societies on Cardiovascular Disease Prevention in Clinical Practice (constituted by representatives of nine societies and by invited experts). Eur Heart J. 2012; 33:1635–701.


9 Lee TG, Hwang MW, Lee SK, Choe BK, Koh BH, Song IB. A Study on the Prevalence and Risk Factors of the Metabolic Syndrome according to Sasang Constitution. Journal of Korean Med. 2006; 27:2. 14–22.


10 Kim DR. A Study on 4 Type Constitution and Life Character of Obese Patients. Journal of Sasang Constitutional Medicine. 1997; 9:1. 303–313.


11 Yang SM. Association between Risk Factors and Prevalence of Metabolic Syndrome according to Sasang Constitution in Wonju Cohort Study. Doc of Sangji Univ. 2007.


12 Choi JW, Yu JS. Cut-off Values of Waist Circumference and Body Mass Index for Metabolic Syndrome according to Sasang Constitution. Journal of Sasang Constitutional Medicine. 2014; 26:4. 365–378.


13 Choi KJ. A Study on the Influence of Sasang Constitution on Insulin Resistance. Doc of Kyunghee Univ. 2010.


14 Lee JW, Jang HS, Park BJ, Lee EJ, Koh BH, Lee JH. Can the Sasang Constitutional Type Trait Act as an Independent Risk Factor for Dyslipidemia? Journal of Korean Med. Obesity Research. 2014; 14:2. 63–71.


15 Kim YH, Park JH, Kwak JY, Park JM, Ahn TW. A Study of the Effect on Obesity in Taeeumin Animal-experimental Model Induced Lung Fibrosis with Bleomycin. Journal of Sasang Constitutional Medicine. 2016; 28:2. 147–162.


16 Park JH, Kim YH, Kwak JY, Hong SJ, Park JM, Ahn TW. A study on the effect on obesity and lipid metabolism in liver hypofunction animal-experimental model induced by Acetaminophen(AAP) injection. Journal of Korean Med. 2016; 37:3. 47–61.


17 Kim KB, Park SO. 2001; A study of the correlation of stress and powerlessness based on hemodialysis patients’ constitution of the Korea. Journal of East-West Nursing Research. 6:1. 7–22.


18 Kim JD. 上壽如水: My Sassang constitution and characteristic 4. pastoral information. 3:4. 2010; 102–104.


19 Kim BH, Joe DY, Lee SH, Park SE, Ga MK. Generation Sasang Constitution Diagnosis and Classification Using Acoustics Analysis Element. Korea information and communications society. 2006; 614–617.


20 Park GS, Han JS, Kim HJ. Effect of Diet Contents on Serum Composition and Urinary Excretion in Female Students according to Sasang Constitution. J East Asian Soc Dietary Life. 1998; 8:3. 271–279.


21 Moon SH, Sin SH, Kim HJ, Kim JY. A Relationship of the Obesity and Body Composition Analysis by Sasang Constitution. Journal of Korean Rehabilitation Med. 2002; 12:4. 1–10.


22 Ucero AC, Benito-Martin A, Izquierdo MC, Sanchez-Niño MD, Sanz AB, Ramos AM, et al. Unilateral ureteral obstruction: beyond obstruction. Int Urol Nephrol. 2014; 46:4. 765–776.


23 Hwang GH, Noh YH, Heo YR. A Study on Hyperlipidemia in Koreans - Specially Related to Hematological Characteristics and Risk Factors of Hypercholesterolemia. Journal of the Korean Society of Food Science and Nutrition. 1999; 28:3. 710–721.


24 Lee HJ, Shin GJ, Park SH, Cho HK. Insulin Resistance and Visceral Fat Obesity in Hyperlipidemia. Korean Circulation Journal. 1999; 29:7. 673–679.


25 Byrne CD, Brindel NPJ, Wang TWM, Hales CN. Interaction of non-esterified fatty acid and insulin in control of triacylglycerol secretion by Hep G2 cells. J Biochem. 1991; 280:99–104.


26 Randle PF, Garland PB, Hales CN, Newsholme EA. The glucose fatty-acid cycle in obesity and maturity onset diabetes mellitus. Ann NY Acad Sci. 1965; 131:324–33.


27 Yoon HJ, Lee YH, Cha BS. Causal Relationship of Non-alcoholic Fatty Liver Disease with Obesity and Insulin Resistance. Journal of Korean Diabetes. 2014; 15:76–81.


28 DeBosch BJ, Kluth O, Fujiwara H, Schürmann A, Moley K. Early-onset metabolic syndrome in mice lacking the intestinal uric acid transporter SLC2A9. Nat Commun. 2014; 5:4642


29 Shin YT, Kim KK, Hwang IC. Clinical Implication of Plasma Uric Acid Level. Korean Journal of Family Medicine. 2009; 30:9. 670–680.


30 Quinones Galvan A, Natali A, Baldi S, Frascerra S, Sanna G, Ciociaro D, et al. Effect of insulin on uric acid excretion in humans. Am J Physiol. 1995; 268:1–5.


31 Eder K, Baffy N, Falus A, Fulop AK. The major inflammatory mediator interleukin-6 and obesity. Inflamm Res. 2009; 58:11. 727–736.


32 Sindhu S, Thomas R, Shihab P, Sriraman D, Behbehani K, Ahmad R. Obesity Is a Positive Modulator of IL-6R and IL-6 Expression in the Subcutaneous Adipose Tissue: Significance for Metabolic Inflammation. PLos One. 2015; 10:7. e0133494


33 Stouthard JM, Romijn JA, Van der Poll T, et al. Endocrinologic and metabolic effects of interleukin-6 in humans. Am J Physiol. 1995; 268:813–319.


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